Displacement chromatography is an ideal chromatographic purification technique to identify and isolate mAb charge variants. Why? The technique has the capability to isolate minor components that you can't see with most other methods. This is particularly beneficial for the analysis, production and purification of monoclonal anitbodies in drug therapy development. With displacement chromatography, it is possible to identify and isolate charge variants. Through the idenfication of minor components, you can then identify which variants affect the characteristics the drug. This is particularly helpful for Quality by Design and quality assurance. (FDA guidance)
How does displacement compare to and differ from elution chromatography?
Displacement chromatography:
- Purifies principal components but also simultaneously concentrates minor components and impurities
- Yields selective enrichment of trace components in complex matrices
- Allows for detection of low-level impurities or close-runnig impurities that are otherwise unobservable by other purification methods (ppm level)
- Uses standard laboratory equipment (i.e. analytical HPLC)
How does displacement differ from elution? The images below illustrated similarities and differences. If the tow images below don't display, please click here for the pdf slides.
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